文章摘要
| 王跃生,王金钱,陈银芳,孙艳荣.折光法快速判断中药柱层析过程中的始终点及其在树脂精制丹参有效部位中的应用[J].江西中医药大学学报,2007,(2):51-53. |
| 折光法快速判断中药柱层析过程中的始终点及其在树脂精制丹参有效部位中的应用 |
| Refractive principle to judge the beginning point and the end point of the process of the traditional Chinese medicine filtered by colume Chromatography rapidly and their application in purification active region of Dan Shen Root with macroporous resin |
| 投稿时间：2007-03-20 |
| DOI： |
| 中文关键词: 折光法,柱层析,丹参有效部位,洗脱液始终点 |
| 英文关键词: Refractive method,colume Chromatography,active region of Dan Shen Root,the beginning point and the end point of elution |
| 基金项目:“十五”国家科技攻关计划(No.2004BA721A41) |
| 王跃生,王金钱,陈银芳,孙艳荣 |
| 中药固体制剂制造技术国家工程研究中心 南昌330006 |
| 摘要点击次数: 4343 |
| 全文下载次数: 4978 |
| 中文摘要: |
| 目的:介绍运用折光原理快速判断中药柱层析过程中始终点的方法及其在大孔树脂精制丹参有效部位的应用。方法:通过丹参提取物经AB-8型大孔吸附树脂精制有效部位,水-30%乙醇-80%乙醇梯度洗脱,间隔取样,在线监测洗脱液折光率并分析变化规律,同时以高效液相色谱法和紫外可见分光光度法分别检测丹酚酸B、丹参萜类成分含量作参比,判断洗脱过程的始点和终点。结果:丹参有效部位精制过程中洗脱的始终点为:水洗终点第4BV(折光率0),即30%乙醇洗脱始点,30%乙醇洗脱终点第8BV(折光率10.5),即80%乙醇洗脱始点,8 |
| 英文摘要: |
| Object:introduce the method applying refractive principle to judge thebeginning point and the end point of the process of the traditional Chinese medicine filtered by colume Chromatography rapidly and their application in purification active region of Dan Shen Root with macroporous resin separation technique.Method:by refining the active region of Dan Shen Root with the AB-8 type of macroporous resin,gradient elution of water-30% alcohol-80% alcohol,interval sampling,monitor the refractive index of eluant on-line,analyze its regularity and judge the beginning point and the end point of the elution process.At the same time,high performance liquid chromatography and ultraviolet-spectrophotometry are usded to measure the contents of salvianolic acid B and Terpene,which can be taken as a reference.Result: the beginning point and the end point of the eluotropic process for the active region of Dan Shen Root: terminal point for water is 4th BV(refractive index:0),but the beginning point for 30% alcohol,terminal point for 30% alcohol is 8th BV(refractive index:10.5),but the beginning point for 80% alcohol,the terminal point for 80% alcohol is 11.5th BV(refractive index: 19.5).Conclusion: this method is not only accurate and reliable,but also convenient and rapid,thus it has very strong practic significance in the process of the traditional Chinese medicine filtered by colume Chromatography. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
|
|