文章摘要
| 张启云,张卓辉,张增珠,徐国良.双黄连注射剂中半抗原物质ELISA检测方法的研究[J].江西中医药大学学报,2009,21(1):39-41. |
| 双黄连注射剂中半抗原物质ELISA检测方法的研究 |
| The Study on ELISA Detection Method of the Semi Antigenic Substance of Shuanghuanglian Injection |
| 投稿时间：2008-11-20 |
| DOI： |
| 中文关键词: 双黄连注射剂；半抗原；ELISA |
| 英文关键词: |
| 基金项目:基金项目：江西省卫生厅基金资助项目(2007A024) |
| 张启云,张卓辉,张增珠,徐国良 |
| 江西中医学院现代中药制剂教育部重点实验室,南昌330004 |
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| 中文摘要: |
| 目的：研究双黄连注射剂中半抗原物质的ELISA检测方法。方法：将双黄连注射剂与正常动物血清体外孵育制备完全抗原，免疫动物获得针对双黄连中过敏性半抗原的特异性抗体，ELISA法检测其效价显示，血清稀释至1∶128时仍为阳性，按优化条件用间接竞争ELISA法测定血清特异性并绘制抑制曲线。结果：得到回归方程I =13.362 lgC + 50.503，相关系数r=0.966 3，检测限为0.93 μg/ml，半数抑制浓度IC50=0.92 mg/ml。结论：本文建立的方法可以很好地用于双黄连注射剂 |
| 英文摘要: |
| Objective：To study the detection of the semi antigenic substance of Shuanghuanglian Injection.Methods：To prepare complete antigen by incubation of Shuanghuanglian Injection and blood serum of normal animal in vitro，the animal was immuned to get the specific antibody that aims directly at the allergia incomplete antigen of Shuanghuanglian Injection.Results：The potency detected by two-site ELISA assay demonstrates positive until the serum diluted to 128 folds，serological specificity detected by the indirect competitive inhibition elisa to draw restrain curve according to the optimium conditions，and the regression equation was I =13.362 lgC + 50.503，coefficient correlation，r=0.966 3，detection limit was 0.93μg/ml，half inhibiting concentration IC50=0.92 mg/ml.Conclusion：This method can be used well in the detection of semi antigenic substance of Shuanghuanglian Injection. |
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