文章摘要
| 朱威,张莲芬,雷楗勇,金坚.羟喜树碱结合肽的结合作用分析[J].江西中医药大学学报,2009,21(1):50-51. |
| 羟喜树碱结合肽的结合作用分析 |
| To search for homologous sequence mode of 10-Hydroxyl camptothecine binding peptides |
| 投稿时间：2008-12-15 |
| DOI： |
| 中文关键词: 羟喜树碱；结合肽；保守性模式 |
| 英文关键词: |
| 基金项目:基金项目：江苏省卫生厅科研资金资助课题(H200625) |
| 朱威,张莲芬,雷楗勇,金坚 |
| 江南大学医药学院分子药理学研究室，江南大学工业生物技术教育部重点实验室,无锡214122 |
| 摘要点击次数: 6817 |
| 全文下载次数: 9567 |
| 中文摘要: |
| 目的：对羟喜树碱结合肽进行结合作用分析，为进一步研究阐明羟喜树碱的抗癌机制、耐药机制以及毒理机制提供帮助。方法：使用生物信息学软件对羟喜树碱结合肽序列进行比对分析，寻找羟喜树碱结合肽同源保守性模式，对羟喜树碱结合肽进行结合作用分析。结果：羟喜树碱结合肽原始序列的保守性模式为xxxxxLxPxxxx。其中L、P皆为疏水脂肪族氨基酸。依据氨基酸可以分为疏水脂肪族氨基酸、疏水芳香族氨基酸、极性带电氨基酸和极性不带电氨基酸的原则，分别以G、F、R、N四个符号来代表四大类氨基酸，对羟喜树碱结合肽原始序列进行了简并转 |
| 英文摘要: |
| Object：To search for homologous sequence mode of 10-Hydroxyl camptothecine(HCPT) binding peptides and their binding mechanism，so as to offer help with researches on decoding the anticancer mechanism of HCPT，the mechanism underlying resistance to HCPT and the mechanism of its side effects.Method：The homologous sequence mode and binding effects of HCPT binding peptides were analyzed by bioinformatical software.Result：The homologous sequence mode of original HCPT binding peptides was identified as xxxxxLxPxxxx while the homologous sequence mode of degeneracy HCPT binding peptides was identified as xGxxGGGNxGGx by sequence alignment analysis.The homologous sequence mode of HCPT binding peptides consisted of hydrophobic amino acid residues.Conclusion：The HCPT binding peptides might form hydrophobic pockets to interact with HCPT and uncharged polar amino acid residues within the hydrophobic pockets would enhance the binding effects between HCPT and HCPT binding peptides by forming hydrogen bonds with HCPT. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
|
|