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文章摘要
尹小明,赵诗云,吴东风,陈厚爱,黄学明,孙敬,王晓莉,曾黎峰,熊美丽,指导:朱大诚.白花蛇舌草提取液诱导多药耐药白血病细胞HL-60/ADR凋亡的研究[J].江西中医药大学学报,2009,21(2):59-62.
白花蛇舌草提取液诱导多药耐药白血病细胞HL-60/ADR凋亡的研究
Study the Inhibitory Action and Inducing Apoptosis of Acetic acid ethyl ester Extracts from Hedyotic diffusa on Multidrug Resistance Leukemic cell line HL-60/ADR
投稿时间:2008-11-03  
DOI:
中文关键词: 白花蛇舌草乙酸乙酯提取液;多药耐药;HL-60/ADR;凋亡
英文关键词: Acetic acid ethyl ester extracts from Hedyotic diffusa;Multidrug resistance; HL-60/ADR、apoptosis
基金项目:基金项目:江西省卫生厅中医药科研基金资助项目(2006A29)
尹小明1,赵诗云2,吴东风2,陈厚爱2,黄学明3,孙敬1,王晓莉1,曾黎峰1,熊美丽1,指导:朱大诚4
1.江西省人民医院,南昌 330006;2.江西省中医药研究院,南昌330077;3.南昌大学第一附属医院,南昌330006;4.江西中医学院,南昌330004
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中文摘要:
      目的:研究白花蛇舌草乙酸乙酯提取液对多药耐药白血病细胞HL-60/ADR的抑制作用及其机制。方法:采用MTT比色试验观察白花蛇舌草乙酸乙酯提取液对多药耐药细胞HL-60/ADR的抑制作用(HL-60/ADR的耐药倍数为204倍)。采用光镜、电镜技术观察细胞形态结构的改变,利用流式细胞术(FCM)检测细胞凋亡率。结果:白花蛇舌草乙酸乙酯提取液能明显抑制HL-60/ADR细胞的生长,药物浓度相当于生药41.67 mg/ml时,即具有显著的抑制作用,抑制率呈剂量和时间依赖性,半数抑制浓度(IC50)相当于生药7
英文摘要:
      Objective: To study the inhibitory action and mechanism of acetic acid ethyl ester extracts from Hedyotic diffusa on multidrug resistance leukemic cell line HL-60/ADR. Methods: To observe the depressant effect of acetic acid ethyl ester extracts from Hedyotic diffusa on multidrug resistance leukemic cell line HL-60/ADR (the drag-fast multiple of HL-60/ADR is 204)by MTT.To observe the change of the cells morphology structure by light microscope and electron microscope,to detect the apoptosis rate by flow cytometry(FCM).Results:The extracts from Hedyotic diffusa could effectively inhibit the proliferation of HL-60/ADRcells.When the concentration is amount to 41.67mg/ml in pharmacognostics.it has effectively inhibitory action.The inhibition rate has dependence of dosage and time.The IC50 is amount to 70.41mg/ml in pharmacognostics.Acetic acid ethyl ester extracts from Hedyotic diffusa could induce apoptosis of multidrug resistance leukemic cell line HL-60/ADR have been confirmed by flow cytometry(FCM),the apoptotic rate has dependence of dosage and time.Conclusion: Hedyotic diffusa could effectively inhibit the proliferation of multidrug resistance leukemic cell line HL-60/ADR,induce HL-60/ADR to apoptosis is one of its mechanism.
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