文章摘要
刘顺根,李邦华,张琦.川芎嗪对小鼠外周血造血干细胞动员作用的研究[J].江西中医药大学学报,2009,21(2):65-68. |
川芎嗪对小鼠外周血造血干细胞动员作用的研究 |
Mobilization of ligustrazine on peripheral blood hematopoietic stem cells in mice |
投稿时间:2008-10-15 |
DOI: |
中文关键词: 川芎嗪;外周血造血干细胞动员作用;小鼠 |
英文关键词: ligustrazine; peripheral blood hematopoietic stem cell; Mobilization |
基金项目: |
刘顺根1,李邦华2,张琦3 |
1.南昌大学第二附属医院二部,南昌330006;2.江西省肿瘤医院内五科,南昌330029; 3.南昌大学研究生院医学部2005级硕士研究生,南昌330006 |
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中文摘要: |
目的:探讨川芎嗪对小鼠外周血造血干细胞的动员作用及其对小鼠外周血细胞和骨髓基质细胞粘附分子的影响。方法:30只BALB/c小鼠,随机分为3组,川芎嗪组(50 mg·kg-1·d-1)、rhG-CSF组(250 μg·kg-1·d-1)、生理盐水组,腹腔注射d1~7,每日1次,第8天,采用外周血WBC、MNC计数、流式细胞术、造血祖细胞体外培养、免疫细胞化学等检测各组给药后对外周血WBC、MNC、CD34+ |
英文摘要: |
Objective: To study ligustrazine for mobilizing peripheral blood hematopoietic stem cells and the effect of its on the expression of adherent molecule of peripheral blood hematopoietic stem cells and bone marrow cells in BABL/c mice.Methods: thirty BABL/c mice were divided into three groups at random(n=10 each):From day 1 to 7,mice were intraperitoneally injected with ligustrazine 50 mg.kg-1.d-1(Group ligustrazine)、and rhG-CSF 250 μg.kg-1.d-1(Group rhG-CSF) respectively,normal control with equivalence saline.On the eighth day,the effects of the numbers of WBC and MNC,the percentage of CD34+ cell and CD49d positive cell and the yields of CFU-GM、CFU-MK、CFU-E in peripheral and the percentage of VCAM-1 positive cells in bone marrow stromal cells of the mice were observed by means of WBC and MNC counts,flow cytometry,hematopoietic cell culture in vitro and immunocytochemistry.Results 7 days after be treated of group ligustrazine,the numbers of WBC and MNC reached the maximum,which were 3.2 times and 3.9 times that of the NS group respectively; the percentage of CD34+ cells and CD49d positive cells in peripheral blood and VCAM-1 positive cells in bone marrow stromal cells in group ligustrazine was(0.86±0.42)%、(12.91±2.84)% and(48.47±7.87)% respectively,were markedly higher than in NS group(P<0.05); the yields of CFU-GM 、CFU-MK and CFU-E in group of tanshinol was (11.70±3.23)%、(11.20±2.88)% and (24.50±8.10)% respectively,were markedly higher than in NS group(P<0.01).Conclusion The results indicate that ligustrazine could mobilize hematopoietic stem cells into peripheral blood.And this role may be related to the increase adhesion molecule expression in peripheral blood cells and mouse bone marrow stromal cell. |
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